Toolbox of Customized Reagents Including Antibodies, Oligonucleotides for qRT-PCR, Plasmids, Lentiviral Vectors, shRNA, and Related Molecular Tools

lists validated qRT-PCR oligonucleotides developed by participating laboratories for use in NRF2-related projects across cellular, animal, and clinical models.
The curated reagents encompass detection and manipulation tools targeting NRF2 (NFE2L2), KEAP1, and multiple downstream effectors including HMOX1 (HO-1), NQO1, GCLM, TXNRD1, SOD2, and GSTA4. Each entry provides validated sequences or clone identifiers, experimental conditions, and references to their use in BenBedPhar-associated publications. This standardization ensures reproducibility and interoperability among partner institutions, facilitating cross-laboratory collaborations and assay comparability.
The activity builds additional scientific advances exemplified by “Molecular pharmacology and translational targeting of the NRF2–KEAP1 axis” (Free Radical Biology and Medicine, 2022), “Novel modulators and chemical probes for NRF2 signaling” (PubMed ID: 36100148), and “Cell-based and molecular tools to dissect NRF2 regulation in health and disease” (PubMed ID: 40043449). These studies demonstrate the growing application of engineered reagents in drug discovery, redox biology, and translational pharmacology.
By consolidating this toolbox, BenBedPhar provides a shared technological infrastructure that accelerates experimental standardization and data generation across the network. The reagents serve as a foundation for ongoing NRF2 pathway validation, mechanistic studies, and preclinical screening of NRF2-targeted therapeutics, directly supporting the Action’s objectives in methodological harmonization and translational readiness.